LOCUS       V002350                 4818 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002350
VERSION     V002350
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     A cryptic plasmid of Lactobacillus casei and construction of shuttle
            vector and food-grade vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2003) Akira Kushiro, Yakult Central Institute for
            Microbiological Research, Clinical Microbiology; 1796, Yaho,
            Kunitachi, Tokyo 186-8650, Japan (E-mail:akira-kushiro@yakult.co.jp,
            Tel:81-42-577-8962(ex.2403), Fax:81-42-577-3020)
REFERENCE   3  (bases 1 to 4818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-SEP-2003) Akira Kushiro, Yakult Central Institute for
            Microbiological Research, Clinical Microbiology"; volume: " 1796,
            Yaho, Kunitachi, Tokyo 186-8650, Japan
            (E-mail:akira-kushiro@yakult.co.jp, Tel:81-42-577-8962(ex.2403),
            Fax"; pages: "81-42-577-3020"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1378..3697
                     /plasmid="pYIT356"
                     /mol_type="other DNA"
                     /db_xref="taxon:1582"
                     /organism="Lactobacillus casei"
     regulatory      176..181
                     /regulatory_class="minus_35_signal"
     regulatory      203..208
                     /regulatory_class="minus_10_signal"
     CDS             264..998
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     1308..1324
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   2056..2076
                     /label="DR1"
                     /note="DR1"
     repeat_region   2065..2080
     repeat_region   2077..2097
                     /label="DR2"
                     /note="DR2"
     repeat_region   2086..2101
     repeat_region   2098..2118
                     /label="DR3"
                     /note="DR3"
     repeat_region   2107..2122
     repeat_region   2119..2139
                     /label="DR4"
                     /note="DR4"
     repeat_region   2128..2143
                     /rpt_unit_range=2128 .. 2143
     regulatory      2152..2157
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /regulatory_class="minus_35_signal"
     regulatory      2175..2180
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /regulatory_class="minus_10_signal"
     CDS             2224..3036
                     /codon_start=1
                     /gene="repB"
                     /product="Replication protein"
                     /label="repB"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="BAD42833.1"
                     /translation="MNVALAGLLDRQDYLVTQANDLARSLGNLSTFQHKVLDYCFSYVT
                     ANDNANTVYHLTTLEIIHHLGLNTSGDSYKRVVKAFKALNENTAIYMRTTEPDGKHGIL
                     MTSLFAYIKVIDDGRVEFKFSATVAPFVFQLKRQFYSFHLSELTRVRSKYTLSMMKLWN
                     ANAIGKWRDQNDPSSLPPAATIKGSLTDWESWFLGSDDDGKPIKWPAGRFKQKAIDVAI
                     KELGSLYPQTMINVLRLTHGRRIEGFQIEFRPIRTVLDLNPQVIDGKP"
     gene            2224..3036
                     /gene="repB"
                     /label="repB"
     primer_bind     complement(3718..3734)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3742..3758)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3766..3796)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3811..3832)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4120..4708)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgcaaaagaa aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa
       61 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg
      121 cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat
      181 agtgcagtat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      241 tgtaattaag aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa
      301 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg
      361 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac
      421 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac
      481 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac
      541 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag
      601 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc
      661 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc
      721 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa
      781 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact
      841 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc
      901 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt
      961 ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt
     1021 aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg tatactactg
     1081 acagcttcca aggagctaaa gaggtcccta gcgtatgcgg tgtgaaatac cgcacagatg
     1141 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     1201 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1261 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1321 cagtgcggcc gcgaattcga gctcggtacc cggggatcct ctagagcatg caagcttgaa
     1381 gctcgccaag taccacgtca aggtcactaa gtcgactatt ttggtggacc tgttagccga
     1441 gcagtaaaac cggcaatcga tcagttgtcg gttgaagcgt ttctgtgcta aaaatatgga
     1501 tacaaacagg gagcaacccc aaaaaagagt gctctcttag cagagccgtt aaaggcagaa
     1561 cagccgcact cccagtatct cgggaatgcg gccgttttgt ctttaatgct attgctggta
     1621 aacgcttcag ctaacaaaac accttaggac gcaaatatgg acttctgaga gcctttcagc
     1681 tgtctgatgt atctctgcag tatccgtatt tgctcgtgaa tggccgtgta agccctccga
     1741 gcccgtaggc gaggtttgtt ctgaaccacg caagcgaagc gcgctaggga aagccagagg
     1801 cgcgcaggcg gagccggagt taaacgtggc gaagccacac ctttttaggg agcgaagcga
     1861 ccaagtgttg gtatggggtt tggggagagg ttctccccaa ggtcttatgt tgtattttta
     1921 cgtggaaaaa gttggctcaa gcgttttttt ttttttgcct cgcgcgtatg taattacatt
     1981 gtcctataca ttttaattac atttaaatac attagggggg cacgaacgct ttaatggcgg
     2041 cgttatcgtg gcactaaaat acctttttcc acgttgaaaa tacttttttc cacgttgaaa
     2101 ataccttttt ccacgttgaa aatacttttt tccacgttga aaatacctta tatgtaatat
     2161 aaacttaaag tgagtatttt aggaattggg ggtatctagt tggcacttac atcaaacaaa
     2221 aaagtgaatg ttgctctggc ggggttattg gatcgtcagg actacctagt aacgcaagcc
     2281 aatgacctgg caagatctct cggtaatttg agtacgttcc agcataaggt gttggattat
     2341 tgctttagtt acgtcacggc taacgacaat gcaaacacgg tgtatcacct gacaacacta
     2401 gagatcattc atcatttagg tcttaacact tccggcgaca gctataaacg tgtggttaag
     2461 gcttttaagg ctttaaatga aaatactgct atatacatgc gtaccacaga gcccgacggt
     2521 aaacacggta tcttaatgac ctcattattc gcgtatataa aagtgataga tgatggtcgt
     2581 gttgagttca aattcagtgc aacagttgct ccgttcgtat ttcagctgaa acgacaattc
     2641 tactcgttcc atctttcgga gctgactaga gttcgttcca aatataccct ctcaatgatg
     2701 aagctgtgga acgcaaatgc tatcggaaaa tggcgtgacc aaaacgatcc tagctctcta
     2761 ccacctgccg ctactattaa gggaagcctt acagattggg agtcctggtt tcttggatct
     2821 gacgatgatg gaaaacctat taagtggccg gcaggtaggt ttaaacaaaa agccatagat
     2881 gttgccatca aggaacttgg ttcactatat cctcaaacca tgattaatgt gttgaggctt
     2941 acacacggcc gacgaatcga aggctttcaa attgaatttc gtcccatccg aacagttctt
     3001 gatttgaatc ctcaagttat tgacggtaaa ccctaggctc tgaactagtg gaagtgcgaa
     3061 agcacacaaa atcccgccaa attctatata tctattgcgc gatgctgtgc accttcaaga
     3121 acgggtgtga ttgggatggt tttccaaatt cattatagaa agattgcagt ccgtaatgaa
     3181 gttccaataa caattagttg taagcgtcaa catagtagta tttataaaat ttactgagtt
     3241 gattgcgaaa gatactgaac gggtcttaga tgaactagac ggcaacacaa ccgctatcct
     3301 gtatgcgcct gaaagcctac cagaggcaca cccatttagc tatcattcac caacttaata
     3361 cagacaactt tccgttatgg aatccgccca gttaacgtaa tggtgcttag cagcagtgct
     3421 cgccccacct ggttcacaac ctaccacttt gtgaatattg aacgagttac cccatagtag
     3481 cgtgccctag tgggttttct aacttgaatg cctagtgaaa tagccacgat agctaccgtg
     3541 gctatttgtt actttccagt gccaaacaat ctctgccaaa aagacggcgg ctgttctgga
     3601 ctactttcag cctttccagg cgcttctggc tctcgttcat tcgtttgttc agaatcactc
     3661 tcaggttcgt cttggggaaa ttctagcttc ttaagctgcg gccgcggcgt aatcatggtc
     3721 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3781 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3841 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     4321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4561 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4801 cttcacctag atcctttt
//
